Development and validation of an eDNA detection workflow for saltwater crocodiles in Northern Territory waters

Awarded 11 Dec 2025

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Awarded11 Dec 2025
Value$246,000.00
StateNT
CategoryResearch & Development

Department of Lands, Planning and Environment awarded a contract to Charles Darwin University for development and validation of a field-ready environmental DNA (eDNA) workflow to detect saltwater crocodile (Crocodylus porosus) in Northern Territory waters and delivery of a statistically defensible sampling scheme for Parks and Wildlife operational use.

The project was based on field collection of samples and laboratory and bioinformatic analysis at CDU and comprised three objectives: (1) assay evaluation and captive trials for eDNA detection of NT C.

porosus; (2) a workshop and co-design on sampling methods for eDNA detection of NT C.

porosus; and (3) development and assessment of an eDNA field sampling scheme at six agreed sites for detection of C.

porosus.

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